Mammalian display

Boghog: consistent citation formatting

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Antibody engineering is commonly carried out by displaying mammalian (e.g., human, mouse) antibody fragments on the surface of microorganisms ([[Phage display|bacteriophage]] or [[Yeast display|yeast]]). Mammalian cells such as human embryonic kidney 293 ([[HEK 293 cells|HEK-293]]) cells can be used to display [[Single-chain variable fragment|single-chain antibody fragments]] (scFvs) on the cell surface.<ref name=":0">Liquid error: wrong number of arguments (given 1, expected 2)</ref> The method is named "mammalian cell display".<ref name=":0" /><ref>Liquid error: wrong number of arguments (given 1, expected 2)</ref><ref>Liquid error: wrong number of arguments (given 1, expected 2)</ref> To prove the principle that affinity maturation is possible in mammalian systems, cells expressing an antibody with high affinity were efficiently enriched from a large excess of cells expressing a lower affinity antibody. Moreover, selection of a combinatory library randomized in an intrinsic antibody hotspot was also successful. Although this approach is challenged by the inherent limitations of library management in mammalian cell culture, development of mammalian cell display will become relevant in biomedical applications.<ref>Liquid error: wrong number of arguments (given 1, expected 2)</ref>

== References ==

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